Figure 3(a) shows that UCA1 was significantly upregulated in SKBR3 (FC = 3.29, p=0.01), MDA-MB-468 (FC = 2.74, p=0.02), MDA-MB-231 (FC = 2.63, p=0.05), and BT474 (FC = 2.12, p=0.04) breast cancer cell lines. A slight increasing trend in UCA1 expression was observed in BT-20 and T47D cell lines (Figure 3(a)). Next, we evaluated the expression of UCA1 in primary breast cancer tissues. Total RNA was isolated from 25 primary tumor-matched normal tissues. High UCA1 expression levels (FC = 3.37, p < 0.0001) were found in breast tumors compared to matched normal tissues (Figure 3(b)). This evidence concerns the gene UCA1 and neoplasm.