FOXP3 and type 1 diabetes mellitus: Interestingly, when compared to healthy individuals, we found a significant decrease in FOXP3+ expression (MFI) of Helios− Tregs (P = 0.0264) (Figure 1D) and an increased frequency of CD25− /low in Helios+ Tregs (P = 0.0109) (Figure 1E) in T1D individuals.