Interestingly, the percentage of Helios+ Tregs in CD4 T cells was positively correlated with T1D disease duration (Spearman r = 0.461, P = 0.0001) and negatively correlated with age at T1D diagnosis (Spearman r = −0.288, P = 0.0209) (Figures 2C, D). This evidence concerns the gene CD4 and type 1 diabetes mellitus.