Previous studies suggested the interaction between DNA methylation and both age and lifestyle (Zykovich et al., 2014; Wilson et al., 2017; Liu et al., 2018; Zhang et al., 2018); we therefore tested the correlation between the methylation levels of ACTB and age, sex, and the status of smoking and drinking in all 147 controls and in all 139 cases who developed stroke within 2 years after enrollment. The gene discussed is ACTB; the disease is stroke disorder.