The methylation level of ACTB_CpG_4.5 was inversely correlated with age in controls (Spearman rho = -0.193, P = 0.020, Figure 4A), but not with age in stroke cases (Supplementary Table 6), which indicated that ACTB_CpG_4.5 methylation might be an age-dependent factor for the risk of stroke. This evidence concerns the gene ACTB and stroke disorder.