TSPO PET imaging studies of MDE from MDD consistently report greater TSPO VT, or similar measures of TSPO binding, in the prefrontal cortex (PFC) and anterior cingulate cortex (ACC), as demonstrated across six different studies at four different sites totaling 142 patients and 93 controls24–28, with ACC values elevated 15–67% and PFC values elevated 25–35%. This evidence concerns the gene TSPO and major depressive disorder.