In particular, there were 37 targets involved in the PI3K-Akt signaling pathway (hsa04151), and the associated targets included AKT1, BDNF, GDNF, GSK3B, PTEN, TP53, VEGFA, etc. The above results pointed out that multiple mechanisms were involved in the pathogenesis of depression. Here, AKT1 is linked to major depressive disorder.