To study the role of KLHL38 in NSCLC, its expression was first evaluated in several cancer cell lines (Fig. 2A), of which A549, H1299, and SK-MES-1 cells had low, high, and intermediate levels of KLHL38, respectively; hence, these cell lines were selected for studying the effects of KLHL38 expression. The gene discussed is KLHL38; the disease is cancer.