Given that the ABR threshold of Tmem43+/KI was significantly higher than that of Tmem43+/+ at 16 kHz (Fig. 5G), the DPOAE results indicate a preserved OHC function in these frequency ranges and reflect the characteristics of nonhair cell–related ANSD rather than OHC-related hearing loss, in the Tmem43KI mice. This evidence concerns the gene TMEM43 and hearing loss disorder.