To comprehensively reveal potential relationships of target genes, we developed a PPI network using the 320 target genes and extracted 15 hubgenes (ATF3, BTG2, DUSP1, EGR1, FGF2, FOSB, GNAI1, GNAO1, GNAZ, GNG7, ITPKB, ITPR1, JUNID, NR4A3, PRKCB); and the differential expressions of the 15 hubgenes were subsequently validated with GC tissues from TCGA and with our GC samples. This evidence concerns the gene BTG2 and gastric cancer.