Forty-one of these genes with differential expressions were reported as CHD-related genes, and we selectively validated nine of them (WNT3, SOX9, PEX19, VIT, CDH11, IGFBP5, HAS2, ENO2, and EGR1) using RT-qPCR (Figure 4D). Here, EGR1 is linked to coronary artery disorder.