Taken together, on the basis of our findings and previous studies, Vitagnus seems to be able to increase the estrogen level in PCOS animals due to its estrogen-like activity, and consequently, down-regulates the elevated level of KISS-1 to normal state,which results in the regulation of the HPG axis (Clarkson et al., 2009 ▶; Gorkem et al., 2018 ▶). The gene discussed is KISS1; the disease is polycystic ovary syndrome.