A prospective study performed using a 44 gene panel testing including patients with breast cancer, colorectal cancer, and other early-onset cancers who have tested negative for pathogenic genes including BACA1/2 showed that 5% of the study population carried pathogenic or highly pathogenic variants of moderate to high penetrance genes like BRCA2, MSH6, CHECK2, ATM, and MUTYH and 87 VUS. This evidence concerns the gene MSH6 and cancer.