PDGFA methylation was subsequently implicated in a novel mechanism of hyperinsulinemia-induced hepatic IR, as in vitro studies confirmed that insulin exposure reduced PDGFA methylation and increased its expression, while the direct exposure of cells to recombinant PDGF-AA interfered with insulin signalling by inhibiting insulin-induced AKT activation (66). This evidence concerns the gene PDGFA and Hyperinsulinemia.