The average relative expression level of BHLHE40 in the HCC group (6.86 ± 4.89) were significantly higher than the non-HCC group (0.62 ± 3.65, P < 0.001) and healthy controls (0.00 ± 3.44, P < 0.001). Here, BHLHE40 is linked to hepatocellular carcinoma.