TNF and Hyperglycemia: Subsequent plot filtering uncovered 6 significant proteins in both intracellular and extracellular HUVECs, 7 significant proteins only in intracellular HUVECs and 40 significant proteins only in extracellular HUVECs, cultured in hyperglycemia and TNFα treatment relative to expression in HUVECs with hyperglycemia alone condition (Fig. 3).