Of the 3 human cell lines, we detected a considerable amount of KRIS proteins in both the cell lysate and supernatant from HUVECs (Supplementary Fig. S1) and, since we aimed to investigate the intracellular and extracellular protein levels exposed to TNFα in the presence of hyperglycemia, HUVECs were selected as the model system in this study. This evidence concerns the gene TNF and Hyperglycemia.