Our results showed that overexpression of EYA2 promoted the proliferation and invasion of breast cancer cells, MCF-7 and lung cancer cells, A549 cells, and revealed a certain inhibitory effect on colon cancer cells, HT-29 and pancreatic cancer cells, PANC-1 cells (Additional file 3: Fig. S3C–E), which was consistent with the previous studies by others [6, 7, 9]. This evidence concerns the gene EYA2 and malignant colon neoplasm.