Among these, expression levels of PAX7 (2.85 e-5 ± 5.75-e-6) > (3.82E-6 ± 1.68E-7), MYOG (2.16E-4 ± 1.16E-4) > (1.36E-5 ± 1.35E-5), PITX1 (8.98 e-5 ± 1.99E-5) > (2.78e-5 ± 5.56E-6), SIX1 (2.57–3 ± 9.52E-5) > (2.16E-4 ± 1.16E-4), SIX4 (8.57 e-5 ± 3.07E-5) > (4.85E-5 ± 6.60E-6) and ITGA7 (4.54E-4 ± 1.26E-4) > (8.24E-5 ± 6.24E-5) in the paralytic esotropia group were significantly greater than that in the control group (Fig. 3). Here, SIX4 is linked to Esotropia.