To further examine the effects of diabetic and non-diabetic plasma on inflammatory cytokine expression in Kupffer cells of diabetes, plasma harvested from Leprdb/db and Lepr+/+ mice was injected into the adipose tissue of Leprdb/db mice, and Kupffer cells were then isolated from the liver of Leprdb/db mice and subjected to Q-PCR analysis to determine mRNA expression of inflammatory cytokines. This evidence concerns the gene LEPR and diabetes mellitus.