Hippocampal neurons were treated with 25 nM BoNT/A(0) or HCC/A for 3 days and the presynaptic vesicle release at individual boutons induced by electrical stimulation measured using synaptophysin pHluorin (SypHy; (Craig et al., 2015, Girach et al., 2013, Shi et al., 2020, Tang et al., 2015). This evidence concerns the gene SYP and hepatocellular carcinoma.