To evaluate TAM frequency, present in tumor microenvironment, we dissociated the tumor mass and stroma on the 22nd day after inoculation and determine the main population expressing the surface marker F4/80 (total macrophages) by flow cytometry, observing no significant differences between Trpa1+/+ and Trpa1-/- inoculated mice (Figures 3A, B). Here, TRPA1 is linked to neoplasm.