CD28 and melanoma: To evaluate the metabolic plasticity and efficiency of these cells to undergo an increased oxidative response while also ramping up the glycolysis, we sorted CD8+ T cells from the spleen of Trpa1+/+ and Trpa1-/- mice, stimulated these cells with immobilized CD3+ and CD28+ antibodies for 30 min and then exposed them to B16-F10 melanoma cells in a 5:1 ratio (5 tumor cells to 1 CD8+ T cell).