In vitro, long-term cultures of primary AML blasts in the presence of AMG330 induced T cell activation, PDL1 over-expression on T-cells, release of IFN-γ, TNF-α, Interleukin-2, IL-10 and IL-6 (64, 65) and reduced bone marrow immune-suppressive CD14+ HLA-DRlow monocytic-like myeloid derived suppressor cells (66). Here, CD274 is linked to acute myeloid leukemia.