Finally, we screened out the common genes between the core genes of ECM-related pathways and DEGs, and then performed co-expression analysis with PLOD1. We found that PLOD1 was positively related to HSPG2, COL6A2, COL4A2, FN1, COL1A1, COL4A1, CD44, COL3A1, COL1A2, SPP1, suggesting that they jointly contribute to the occurrence and development of gliomas. This evidence concerns the gene COL4A2 and central nervous system cancer.