Additionally, the GO and GSEA analysis verified that the mechanism of PLOD1’s oncogenic effect was related to ECM, the co-expression analysis revealed that PLOD1 was positively correlated with HSPG2, COL6A2, COL4A2, FN1, COL1A1, COL4A1, CD44, COL3A1, COL1A2 and SPP1. All of them were ECM related genes and the expression of these genes was also correlated with the prognosis of glioma. The gene discussed is SPP1; the disease is central nervous system cancer.