As the trispecific antibody is intended to facilitate an elevated tumor specificity due to simultaneous binding to EGFR and PD-L1 compared to the bsAb solely targeting EGFR, cell-binding experiments were performed on EGFR/PD-L1 double-positive A431 cells using flow cytometry as described before (55) (Supplementary Figure 16A). This evidence concerns the gene CD274 and neoplasm.