To assess the effect of FL (200 ng/ml) on 8 Gy IR-induced DNA damage and repair in ESCC cells, we used immunofluorescence to detect the number of gamma-H2AX (γ-H2AX) at different time points (0, 2, 6, and 24 h) after FL administration in the ionizing radiation X-ray exposure group. Here, H2AX is linked to esophageal squamous cell carcinoma.