The number of tumor lung metastases was calculated, and showed to increase after HCC-exo treatment while further enhanced by restored DLX6-AS1 from HCC-exo (Fig. 11b) In summary, HCC-exo DLX6-AS1 induced M2 macrophage polarization in vivo to promote lung metastasis of HCC cells. The gene discussed is DLX6; the disease is hepatocellular carcinoma.