Given that DKC1125 increased the suppressive effect of 5-FU on cell survival and motility in KITENIN-overexpressed CRC cells, we investigated the in vivo effect of DKC1125 on distant metastasis of CRC in a mouse colorectal liver metastasis model with combinatorial strategy: either alone or in combination with 5-FU. The gene discussed is VANGL1; the disease is colorectal carcinoma.