In order to validate the role of ZEB1-driven EMT in the establishment of the EpCAMlo subpopulation of colon cancer cells in a cell line-unrelated in vivo model mimicking the course of events observed in colon cancer patients, we employed mouse intestinal organoids carrying specific mutations at the Apc, Kras, and Tp53 genes (Apcfl/fl::KrasG12D/+::Trp53fl/R172H; AKP) (Fumagalli et al., 2017). Here, APC is linked to malignant colon neoplasm.