To evaluate whether endothelial cells from an Aß-accumulating AD mouse model could suffer NPA, we defined a gene set (GS) containing the genes upregulated in the retina of Dll4 heterozygous mice (Dll4+/–_Up)34, a model of NPA7–10, and estimated its contribution to the DE genes between APP-PSEN1/+ and WT endothelial cells using GS enrichment analysis (GSEA). Here, DLL4 is linked to Alzheimer disease.