To explore whether the application of notropane alkaloids may reduce the induced apoptotic cell death related to hyperinsulinemia (HI) and hyperglycaemia (HG) condition in HepG2 cells, Tox8 test, Annexin V & Dead Cell assay as well as gene expression analysis of pro-apoptotic factors have been carried out for the investigation of apoptotic cell death in cultured cells. The gene discussed is ANXA5; the disease is Hyperinsulinemia.