With the gradual increase in WHtR, the proportion of male participants gradually increased, the age, height, weight, BMI, WC, SBP, and DBP of all participants showed a gradual increase, the NAFLD patients, smokers and drinkers, and the levels of ALT, AST, GGT, TC, TG, HbA1c and FPG were also on the increased (all Ptrend < 0.05). The gene discussed is GPT; the disease is metabolic dysfunction-associated steatotic liver disease.