When we compared resected pT3 tumours with and without pathological nodal involvement (pT3N0M0, two tumours versus pT3N1M0, four tumours), we observed a stronger pAkt Substrate IHC staining, indicative of PI3K/Akt activity, in the primary site that are associated with nodal involvement. The gene discussed is PIK3CB; the disease is neoplasm.