As this TALK-1 mutation causes MODY in a dominant manner, we also measured the TALK-1 current under heterozygous conditions by creating a TALK-1-WT-P2A-TALK-1-Leu114Pro construct, which allows for equivalent expression of the TALK-1 WT and TALK-1 Leu114Pro subunits. Here, KCNK16 is linked to MODY.