To understand the importance of phospho-eIF4E in bladder cancer initiation, WT, eIF4ES209A/+, and eIF4ES209A/S209A mice were treated with BBN and all were euthanized at 9 or 15 weeks, which corresponds to the initiation of precancerous lesions and tumors, respectively. This evidence concerns the gene EIF4E and urinary bladder cancer.