In order to further verify the functions of DEPDC1B in HCC lines, investigate the functions of CDK1 knockdown in HCC lines, and find the interaction between CDK1 and DEPDC1B, LV-NC(OE+KD), LV-DEPDC1B+NC(KD), LV-shCDK1+NC(OE), and LV-DEPDC1B+shCDK1 were transfected into SK-HEP-1 cell line. Here, CDK1 is linked to hepatocellular carcinoma.