To determine the biological function of ABI1-TSV-11 in LsCC, we first analyzed the expression of ABI1-TSV-11 in a normal colorectal epithelial cell line (CRL-1541) and three CRC cell lines (LoVo, SW480, SW620) by next-generation sequencing (Fig. 2a), and LoVo and SW480 cell lines, which originate from LsCC and have low endogenous ABI1-TSV-11 expression and different invasiveness, were then selected, to construct the stable ABI1-TSV-11 overexpressed cell models. The gene discussed is ABI1; the disease is colorectal carcinoma.