To verify if MDR1 and ABCG2 play critical roles in DOX efflux in LCSCs, we incubated HCC cells with DOX plus valspodar or ko143 to examine if such treatments lead to increased DOX accumulation in the bulk and the EpCAM+–CD133+ HCC cells using flow cytometry. This evidence concerns the gene PROM1 and hepatocellular carcinoma.