In sum, these findings revealed that reintroduction of PPDPF hindered HFD-induced NAFLD development in PPDPF-LKO mice, while PPDPF mut lost the rescue effect, indicating that the interaction between PPDPF and Raptor was important for the regulation of mTOR signaling and lipid metabolism by PPDPF. Here, RPTOR is linked to metabolic dysfunction-associated steatotic liver disease.