Thus, to further investigate whether TUG1 regulated Wnt/β-catenin pathway to mediate the progression of CRC, the expression levels of proteins (β-catenin, c-Myc, Bcl-2 and E-cadherin) associated with Wnt/β-catenin pathway in CRC cells transfected with si-TUG1, si-TUG1 + anti-miR-542-3p or si-TUG1 + pc-DNA-TRIB2, as well as the corresponding controls, were measured by western blot. The gene discussed is MYC; the disease is colorectal carcinoma.