To study this therapeutic regimen in an orthotopic tumor type, we treated mice harboring EMT6 breast tumors orthotopically grown in the mammary fat pad with PBS, anti-PD-L1 (atezolizumab), anti-TGF-β, or anti-PD-L1 (atezolizumab) plus anti-TGF-β. Here, CD274 is linked to neoplasm.