The steps in the cancer-immunity cycle were enhanced in PTCH1-mutated tumors in the entire TCGA COADREAD cohort and its MSI-H subgroup (Fig. 3e, g), indicating that the mutation of PTCH1 in CRC potentially facilitates immune cell infiltration, partially accounting for the better response to ICIs in the PTCH1 mutation subgroup. The gene discussed is PTCH1; the disease is colorectal carcinoma.