SLC30A8 and amyloidosis: To determine the effects of lack of ZnT8 in β cells on islet amyloid deposition, we stained pancreatic sections with thioflavin S. After 8–10 weeks of HFD, ZnT8B–/– hIAPP mice compared with hIAPP controls showed significantly elevated amyloid severity measured as the total amyloid per islet area and amyloid prevalence measured as the number of islets positive for amyloid staining (Figure 4, A–C).