Four hours after administration of TTQ-PEG-c(RGD) and a control nanoparticle, TTQ-TF-PEG-NH2, separately into murine thrombosis-induced models in vivo, a ∼7.6-fold relative difference in the NIR-II fluorescence emission intensities between the bilateral external jugular veins was attained when compared to the control group that received TTQ-TF-PEG-NH2. Here, TF is linked to Venous thrombosis.