To identify essential genes involved in the progression of colorectal cancer, we developed a high‐throughput shRNA library screening strategy.[17, 18] The Network Essentiality Scoring Tool (NEST) was used for the negative selections.[19] Several candidate proliferation‐related genes, including DKC1 and five well‐known tumor growth‐associated genes (PCNA, AKT1, HRAS, ERBB2, and KRAS), were identified as top hits (Figure1A). This evidence concerns the gene KRAS and colorectal cancer.