When the BsAb sequence is added after the EGFRvIII CAR-T plasmid sequence, CAR-T cells can target EGFRvIII-positive tumor cells, and the released BsAb can recruit T cells to form a bridge, thereby killing EGFR-positive tumor cells that have lost the EGFRvIII antigen. The gene discussed is EGFR; the disease is neoplasm.