Using a Venn diagram to visualize the down-regulated transcripts in acne samples compared to healthy ones and in the EGF-treated and EGF+PA-co-treated SZ95 sebocytes compared to untreated controls, a possible contribution of 34 significantly altered transcripts from EGF-treated and an additional 41 transcripts from the EGF+PA-co-treated sebocytes to acne pathogenesis could be detected (Figure 7A), with a possible role in the differentiation and lipid metabolism of sebocytes (Figure 7B). The gene discussed is EGF; the disease is acne.