The results showed that the expression levels of 11 target genes, UBE2D3, RNF169, FBXO33, RAD23B, NEURL1B, PCNP, TULP4, KLHL15, RNF34, DERL1, HSP90B1, were upregulated in the METH-induced CPP group when compared to the control group (n = 8, P < 0.05), while the expression of 5 target genes, RNF145, KLHL5, CAND1, KLHL2, HSPA5, did not change significantly (n = 8, P > 0.05) (Figure 8). This evidence concerns the gene KLHL15 and central precocious puberty.