To further determine the involvement of the TrkB/ERK/CREB signaling pathway in protecting neurons against chronic progressive ischemia in hippocampus, hippocampal neurons were cultured, as well as two specific inhibitors which were administrated with or without oxygen glucose deprivation (OGD) model, which is extensively used to simulate ischemia in vivo (Yang et al., 2019). This evidence concerns the gene CREB1 and ischemia.