Results of western blot showed that FEZF1-AS1 overexpression induced EMT in HCC cells with increased expression levels of ICAM1 and Vimentin and decreased expression level of E-cadherin (Figure 3C, 3D), while downregulation of FEZF1-AS1 decreased ICAM1 and Vimentin expression and increased E-cadherin expression. This evidence concerns the gene VIM and hepatocellular carcinoma.