LGALS1 and neoplasm: To demonstrate that fibroblast-derived Gal-1 is directly implicated in tumor cell migration, we set up a wound healing assay in which PANC-1 and DU145 cells, labeled with CellTrackerTM Orange Dye, were seeded in co-culture with AHFs, expressing GFP and stably silenced or not for Gal-1 (Fig. 5A, D).