In summary, our results suggest that one of the tumor-suppressive effects of increased expression of miR-30d is to inhibit RUNX1 and inactivate the aerobic glycolysis signaling, forming a vigorous YTHDC1-miR-30d-RUNX1-SLC2A1/HK1 axis to repress PDAC occurrence and progression. Here, HK1 is linked to neoplasm.