CDK4 and neoplasm: Western blot analysis of extracted tumor tissue also demonstrated that tumors derived from cells with silencing of either Runx1t1 or RBM24 exhibited significantly downregulated Runx1t1, RBM24, and CDK4 levels compared to tumors derived from control cells, and this downregulation was further enhanced by the simultaneous knockdown of both Runx1t1 and RBM24 (Fig. 7d).